HLA Testing for Repeated Pregnancy Losses/ Recurrent IVF Failures

Doctors often recommend a human leukocyte antigen (HLA) compatibility test to couples who have multiple failed IVF cycles where the PGS-tested (genetic testing to detect chromosomal abnormalities) embryos have also failed to implant successfully. HLA compatibility testing can help identify potential rejection issues.

Benefits of HLA testing:

• Certain genetic factors can lead the body to reject embryos, especially when the partners are closely related (for example, first or second cousins).
• Suspected HLA incompatibility. Doctors conduct genotyping of both partners to evaluate compatibility.
• HLA testing is now used in IVF to select only the healthiest embryos for transfer. Standard IVF procedures are then used to transfer the embryo, increasing the chances of a successful pregnancy.

Thus, by combining LIT therapy and HLA testing, doctors can enhance pregnancy outcomes for women who have had repeated pregnancy losses or failed IVF cycles.

Recurrent Pregnancy Loss Treatment

One of the effective treatments to treat recurrent pregnancy loss is using lymphocyte immunisation therapy (LIT). The LIT helps the female partner’s body to develop antibodies to her partner’s sperm and recognise them as safe, thereby reducing the chances of embryo rejection and allowing full-term pregnancy.

Before starting the LIT therapy, doctors usually perform a pre-LIT work-up. This typically includes a comprehensive review of the medical history that includes the timing and frequency of previous miscarriages and immunological assays to determine rejection levels.

The LIT procedure is as follows:

• Blood from the male partner is extracted to separate the white blood cells (WBCs).
• These WBCs are injected into the female partner’s body at multiple locations to generate an immunological response.
• This enables the female body’s immune system to recognise sperm and embryo as non-threatening, resulting in successful conception.

The total number of LIT sessions required differs from person to person, based on the severity of immunologic rejection. Some women require as many as three sittings to get an effective immunisation.

However, there are certain cases where the female body rejects the male partner’s sperm entirely. In such cases, donor blood of

the same blood type can be used for LIT. Once the female body's immune system gets trained to accept donor cells, LIT therapy can be started. Some women may need several LIT sessions prior to embryo transfer.

Dr. Jayesh Amin—the best doctor for recurrent pregnancy loss at Nova IVF Fertility and one of India's leading specialists in LIT—has successfully treated 250+ cases using LIT treatment. With his LIT therapy, women have become pregnant and successfully delivered full term.

If you have experienced multiple pregnancy losses in the past, then visit Nova IVF Fertility’s recurrent miscarriage centre today for effective solutions!

Targeted Treatments to Treat Underlying Cause

• If antiphospholipid antibodies (APLA) are identified, low-dose aspirin and blood-thinning injections can improve pregnancy outcomes. 
• Uterine problems such as a malformed womb or fibroids may necessitate minor surgery. 
• Hormonal abnormalities (such as thyroid issues and diabetes) are treated with drugs or lifestyle modifications. 
• Progesterone supplements may aid women with recurrent miscarriages who experience early pregnancy bleeding. 
• Lifestyle changes, such as weight control and stress reduction, are also important. Always visit an expert for tailored care based on your specific requirements.     
   

Immunomodulatory Treatments for Multiple Pregnancy Loss

One of the effective advanced treatments to treat recurrent pregnancy loss is lymphocyte immunisation therapy (LIT). The LIT helps the female partner’s body to develop antibodies to her partner’s sperm and recognise them as safe, thereby reducing the chances of embryo rejection and allowing full-term pregnancy.

Before starting the LIT therapy, doctors usually perform a pre-LIT work-up. This typically includes a comprehensive review of the medical history that includes the timing and frequency of previous miscarriages and immunological assays to determine rejection levels.

The LIT procedure is as follows:

• Blood from the male partner is extracted to separate the white blood cells (WBCs). 
• These WBCs are injected into the female partner’s body at multiple locations to generate an immunological response.
• This enables the female body’s immune system to recognise sperm and embryo as non-threatening, resulting in successful conception.

The total number of LIT sessions required differs from person to person, based on the severity of immunologic rejection. Some women require as many as three sittings to get an effective immunisation.

However, there are certain cases where the female body rejects the male partner’s sperm entirely. In such cases, donor blood of the same blood type can be used for LIT. Once the female body's immune system gets trained to accept donor cells, LIT therapy can be started. Some women may need several LIT sessions prior to embryo transfer.

Other immunomodulatory therapies for recurrent pregnancy loss include:

• Intralipid therapy
• Granulocyte colony-stimulating therapy (G-CSF)
• Intravenous (IV) or intramuscular (IM) immunoglobulin therapy (IVIG/IMIG)

These treatments function by modulating immune responses to promote the implantation of embryos and pregnancy outcomes.